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Fungal-Grade Reagents and Materials for Molecular Analysis

  • Michael G. LorenzEmail author
  • Michael Lustig
  • Marina Linow
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.

Key words

Fungal DNA Contamination Decontamination DNA-free Fungal PCR reagents Fungal DNA extraction Fungal assays 

Notes

Acknowledgement

The work was supported by the European Union, FP7-HEALTH-2013-INNOVATION-1, project ID 602125: Optimized Diagnostics for Improved Treatment Stratification in Invasive Fungal Diseases (FUNGITECT).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Michael G. Lorenz
    • 1
    Email author
  • Michael Lustig
    • 1
  • Marina Linow
    • 1
  1. 1.Molzym GmbH & Co. KGBremenGermany

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