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Immune Cell-Supplemented Human Skin Model for Studying Fungal Infections

  • Andreas Kühbacher
  • Kai Sohn
  • Anke Burger-Kentischer
  • Steffen RuppEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

Human skin is a niche for various fungal species which either colonize the surface of this tissue as commensals or, primarily under conditions of immunosuppression, invade the skin and cause infection. Here we present a method for generation of a human in vitro skin model supplemented with immune cells of choice. This model represents a complex yet amenable tool to study molecular mechanisms of host–fungi interactions at human skin.

Key words

3D skin model Immune reaction to fungi Host–fungi interaction Infection model 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Andreas Kühbacher
    • 1
  • Kai Sohn
    • 1
    • 2
  • Anke Burger-Kentischer
    • 1
  • Steffen Rupp
    • 3
    • 4
    Email author
  1. 1.Fraunhofer Institute for Interfacial Engineering and BiotechnologyStuttgartGermany
  2. 2.Institute of Interfacial Process Engineering and Plasma TechnologyUniversity of StuttgartStuttgartGermany
  3. 3.Fraunhofer Institut für Grenzflächenund BioverfahrenstechnikStuttgartGermany
  4. 4.Institut für Grenzflächenverfahrenstechnik undPlasmatechnologieUniversität StuttgartStuttgartGermany

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