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Molecular Detection of Resistance to Azole Components

  • Brunella PosteraroEmail author
  • Antonietta Vella
  • Elena De Carolis
  • Maurizio Sanguinetti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

Fungal infections have increased significantly in the last few years, and their outcomes are in part complicated by the emergence of antifungal drug-resistant pathogens. Together with Candida species, the mould Aspergillus fumigatus is one of the most prevalent organisms to cause invasive fungal disease. The molecular detection of (tri)azole resistance in both Candida and Aspergillus species may represent a useful means of monitoring the incidence of clinical isolates with antifungal resistance-associated gene alterations. Here, we describe molecular methods that have been developed to allow for accurate detection of azole-resistant isolates among C. glabrata and A. fumigatus fungal species.

Key words

Antifungal drug resistance Fungal pathogens Molecular analysis Quantitative real-time RT-PCR Gene sequencing 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Brunella Posteraro
    • 1
    Email author
  • Antonietta Vella
    • 2
  • Elena De Carolis
    • 2
  • Maurizio Sanguinetti
    • 2
  1. 1.Section of Hygiene, Institute of Public HealthUniversità Cattolica del Sacro CuoreRomeItaly
  2. 2.Institute of MicrobiologyUniversità Cattolica del Sacro CuoreRomeItaly

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