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The Molecular Blueprint of a Fungus by Next-Generation Sequencing (NGS)

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Human Fungal Pathogen Identification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1508))

Abstract

Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation.

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Author information

Authors and Affiliations

  1. Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany

    Christian Grumaz, Philipp Kirstahler & Kai Sohn

  2. Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, Stuttgart, Germany

    Kai Sohn

Authors
  1. Christian Grumaz
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  2. Philipp Kirstahler
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  3. Kai Sohn
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Corresponding author

Correspondence to Kai Sohn .

Editor information

Editors and Affiliations

  1. Children’s Cancer Research Institute (CCRI), St.Anna Kinderkrebsforschung, Vienna, Austria

    Thomas Lion

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Grumaz, C., Kirstahler, P., Sohn, K. (2017). The Molecular Blueprint of a Fungus by Next-Generation Sequencing (NGS). In: Lion, T. (eds) Human Fungal Pathogen Identification. Methods in Molecular Biology, vol 1508. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6515-1_21

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  • DOI: https://doi.org/10.1007/978-1-4939-6515-1_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6513-7

  • Online ISBN: 978-1-4939-6515-1

  • eBook Packages: Springer Protocols

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