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Genus- and Species-Specific PCR Detection Methods

  • Jan Springer
  • Jürgen LöfflerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

PCR-based detection of fungal pathogens offers a sensitive and specific tool for the diagnosis of invasive fungal infections. A large variety of different clinical specimen types can be used as original material. However, certain precautions, in addition to the published MIQE guidelines [1], need to be taken to prevent contaminations from airborne fungal spores and PCR reagents. In addition, the European Aspergillus PCR Initiative (EAPCRI) recently defined standards for Aspergillus PCR [2, 3], following these recommendations leads to superior sensitivity. The combination of fungal PCR with the galactomannan ELISA assay increases the sensitivity for the detection of Aspergillus DNA from blood, compared to a single assay only [4, 5].

Key words

DNA Detection Fungi PCR Amplification Broad-range PCR Species-specific method 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Medizinische Klinik IIUniversitätklinikum WürzburgWürzburgGermany

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