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Prerequisites for Control of Contamination in Fungal Diagnosis

  • Stefan Czurda
  • Thomas LionEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

Nucleic acid amplification methods facilitate rapid and sensitive detection of clinically relevant fungal pathogens, and can be employed using a variety of patient specimens. However, contamination from various exogenous sources constitutes a serious threat to the validity of amplification-based fungal assays. In this chapter, common origins of fungal contaminants that compromise molecular fungal testing are described, and measures for preventing contamination are proposed. Detailed guidelines for sample handling, reagent selection, contamination screening, and decontamination procedures are provided.

Key words

PCR reagent contamination Contamination prevention DNase-mediated decontamination Invasive fungal infection Molecular fungal diagnostics 

Notes

Acknowledgements

The work presented was conducted within the FUNGITECT project supported by a grant from the European Commission (N° 602125) within the 7th Framework Programme (FP7).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Children’s Cancer Research Institute (CCRI)St. Anna KinderkrebsforschungViennaAustria
  2. 2.LabDia Labordiagnostik GmbHViennaAustria
  3. 3.Department of PediatricsMedical University of ViennaViennaAustria

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