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Isolation of Nucleic Acids for Fungal Diagnosis

  • P. Lewis WhiteEmail author
  • Rosemary A. Barnes
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

PCR can aid in the diagnosis of invasive fungal disease (IFD). While the large number of “in-house” methodologies drives technological diversity, providing robustness, they make it difficult to identify optimal strategies, limiting standardization, and widespread acceptance. No matter how efficient, PCR utility will be limited by the quality of extracted nucleic acid. This chapter highlights benefits and limitations affecting the nucleic acid extraction process, before focusing on recent recommendations that through multicenter evaluation have provided optimal and standardized methodology.

Key words

Sample type DNA extraction Fungal infection 

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© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Public Health Wales Microbiology CardiffUniversity Hospital of WalesCardiffUK
  2. 2.Department of Infection and ImmunityCardiff UniversityCardiffUK

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