Abstract
Alginate encapsulation of cells is an attractive technique in which alginate becomes polymerized entrapping the cells. The structure of formed microbeads/microcapsules is semipermeable as it allows oxygen and nutrients to go in, and waste products and other materials produced by the cells to go out. Here, we describe basic protocols for alginate encapsulation of human hepatocytes and methods for assessing the microbeads produced.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Mitry RR (2009) Isolation of human hepatocytes. In: Hughes RD, Dhawan A (eds) Hepatocyte transplantation, vol 481, 1st edn, Methods in molecular biology. Springer, New York, pp 17–23
Jitraruch S, Dhawan A, Hughes RD, Filippi C, Soong D, Philippeos C et al (2014) Alginate microencapsulated hepatocytes optimised for transplantation in acute liver failure. PLoS One 9(12):e113609
Mitry RR, Hughes RD, Bansal S, Lehec SC, Wendon JA, Dhawan A (2005) Effects of serum from patients with acute liver failure due to paracetamol overdose on human hepatocytes in vitro. Trans Proc 37(5):2391–2394
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Mitry, R.R., Jitraruch, S., Iansante, V., Dhawan, A. (2017). Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads. In: Stock, P., Christ, B. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 1506. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6506-9_19
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6506-9_19
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6504-5
Online ISBN: 978-1-4939-6506-9
eBook Packages: Springer Protocols