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Hepatocyte Transplantation pp 273–281Cite as

Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1506))

Abstract

Alginate encapsulation of cells is an attractive technique in which alginate becomes polymerized entrapping the cells. The structure of formed microbeads/microcapsules is semipermeable as it allows oxygen and nutrients to go in, and waste products and other materials produced by the cells to go out. Here, we describe basic protocols for alginate encapsulation of human hepatocytes and methods for assessing the microbeads produced.

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References

  1. Mitry RR (2009) Isolation of human hepatocytes. In: Hughes RD, Dhawan A (eds) Hepatocyte transplantation, vol 481, 1st edn, Methods in molecular biology. Springer, New York, pp 17–23

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  2. Jitraruch S, Dhawan A, Hughes RD, Filippi C, Soong D, Philippeos C et al (2014) Alginate microencapsulated hepatocytes optimised for transplantation in acute liver failure. PLoS One 9(12):e113609

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  3. Mitry RR, Hughes RD, Bansal S, Lehec SC, Wendon JA, Dhawan A (2005) Effects of serum from patients with acute liver failure due to paracetamol overdose on human hepatocytes in vitro. Trans Proc 37(5):2391–2394

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Author information

Authors and Affiliations

  1. Institute of Liver Studies, King’s College Hospital, Bessemer Road, London, SE5 9PJ, UK

    Ragai R. Mitry, Suttiruk Jitraruch, Valeria Iansante & Anil Dhawan

Authors
  1. Ragai R. Mitry
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  2. Suttiruk Jitraruch
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  3. Valeria Iansante
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  4. Anil Dhawan
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Corresponding author

Correspondence to Ragai R. Mitry .

Editor information

Editors and Affiliations

  1. Department of Surgery, University of Leipzig, Leipzig, Germany

    Peggy Stock

  2. Department of Surgery, University of Leipzig, Leipzig, Germany

    Bruno Christ

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Cite this protocol

Mitry, R.R., Jitraruch, S., Iansante, V., Dhawan, A. (2017). Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads. In: Stock, P., Christ, B. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 1506. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6506-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6506-9_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6504-5

  • Online ISBN: 978-1-4939-6506-9

  • eBook Packages: Springer Protocols

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