Abstract
For the purpose of clinical research of hepatocyte transplantation, procedures for isolation, cryopreservation, thawing, and functional assessment of hepatocytes are described. Although demands for human hepatocytes are increasing in not only cell therapy but also drug development, it is highly difficult to obtain good lots of hepatocytes from human liver tissue. This chapter describes essential issues such as alleviation of warm ischemia, prevention of shear stress, optimization of cryopreservation, and functional assessment, along with securement of quality. All procedures described here are compliant with good manufacturing procedure (GMP) in cell processing facility, approved by the act on measures to ensure safety of regenerative medicine and ethical regulations in Japan.
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References
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Acknowledgement
The author expresses sincere gratitude to all persons who donated liver tissue. The author would also like to thank Dr. Mureo Kasahara who proposed use of remnant liver tissues from living donor liver graft as a source of hepatocytes for transplantation.
The author declares no conflict of interest; no personal or financial interests and no source of financial support from any industry for this study and the manuscript.
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Enosawa, S. (2017). Isolation of GMP Grade Human Hepatocytes from Remnant Liver Tissue of Living Donor Liver Transplantation. In: Stock, P., Christ, B. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 1506. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6506-9_16
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DOI: https://doi.org/10.1007/978-1-4939-6506-9_16
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