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Deep Digging: Far Red Imaging for the Monitoring of Transplanted Hepatocytes in Rats

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Hepatocyte Transplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1506))

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Abstract

Technologies for in vivo imaging of the distribution and integration of cell transplants gain significance for the use of novel cell therapy approaches in regenerative medicine. Applied to adequate animal models, they provide information on the spatio-temporal engraftment and functional performance of the cells transplanted. This chapter includes a detailed description of the in vivo tracking of transplanted hepatocytes in rat liver including the conjugation of antibodies to fluorochromes for far red imaging using a multispectral optical imager.

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References

  1. Hansel MC, Gramignoli R, Skvorak KJ, Dorko K, Marongiu F, Blake W et al (2014) The history and use of human hepatocytes for the treatment of liver diseases: the first 100 patients. Curr Protoc Toxicol 62(14):14 12 11–14 12 23. doi:10.1002/0471140856.tx1412s62

    Google Scholar 

  2. Dhawan A (2015) Clinical human hepatocyte transplantation- current status and challenges. Liver Transpl 21:S39–S44. doi:10.1002/lt.24226

    Article  PubMed  Google Scholar 

  3. Forbes SJ, Gupta S, Dhawan A (2015) Cell therapy for liver disease: from liver transplantation to cell factory. J Hepatol 62(1 Suppl): S157–S169. doi:10.1016/j.jhep.2015.02.040

    Article  CAS  PubMed  Google Scholar 

  4. Hoyer DP, Paul A, Gallinat A, Molmenti EP, Reinhardt R, Minor T et al (2015) Donor information based prediction of early allograft dysfunction and outcome in liver transplantation. Liver Int 35(1):156–163. doi:10.1111/liv.12443

    Article  PubMed  Google Scholar 

  5. Hilger I, Leistner Y, Berndt A, Fritsche C, Haas KM, Kosmehl H et al (2004) Near-infrared fluorescence imaging of HER-2 protein over-expression in tumour cells. Eur Radiol 14(6):1124–1129. doi:10.1007/s00330-004-2257-9

    Article  PubMed  Google Scholar 

  6. Bloch S, Lesage F, McIntosh L, Gandjbakhche A, Liang K, Achilefu S (2005) Whole-body fluorescence lifetime imaging of a tumor-targeted near-infrared molecular probe in mice. J Biomed Opt 10(5):054003. doi:10.1117/1.2070148

    Article  CAS  PubMed  Google Scholar 

  7. Koenig S, Krause P, Hosseini AS, Dullin C, Rave-Fraenk M, Kimmina S et al (2009) Noninvasive imaging of liver repopulation following hepatocyte transplantation. Cell Transplant 18(1):69–78

    Article  PubMed  Google Scholar 

  8. Caysa H, Hoffmann S, Luetzkendorf J, Mueller LP, Unverzagt S, Mader K et al (2012) Monitoring of xenograft tumor growth and response to chemotherapy by non-invasive in vivo multispectral fluorescence imaging. PLoS One 7(10):e47927. doi:10.1371/journal.pone.0047927

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Mansfield JR, Gossage KW, Hoyt CC, Levenson RM (2005) Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging. J Biomed Opt 10(4):41207. doi:10.1117/1.2032458

    Article  CAS  PubMed  Google Scholar 

  10. Thompson NL, Hixson DC, Callanan H, Panzica M, Flanagan D, Faris RA et al (1991) A fischer rat substrain deficient in dipeptidyl peptidase IV activity makes normal steady-state RNA levels and an altered protein. Use as a liver-cell transplantation model. Biochem J 273(Pt 3):497–502

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Laconi E, Oren R, Mukhopadhyay DK, Hurston E, Laconi S, Pani P et al (1998) Long-term, near-total liver replacement by transplantation of isolated hepatocytes in rats treated with retrorsine. Am J Pathol 153(1): 319–329. doi:10.1016/S0002-9440(10)65574-5

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Stock P (2014) Age-dependent hepatocyte transplantation for functional liver tissue reconstitution. Methods Mol Biol 1213:41–50. doi:10.1007/978-1-4939-1453-1_4

    Article  CAS  PubMed  Google Scholar 

  13. Hartel-Schenk S, Gossrau R, Reutter W (1990) Comparative immunohistochemistry and histochemistry of dipeptidyl peptidase IV in rat organs during development. Histochem J 22(10):567–578

    Article  CAS  PubMed  Google Scholar 

  14. Watanabe Y, Kojima T, Fujimoto Y (1987) Deficiency of membrane-bound dipeptidyl aminopeptidase IV in a certain rat strain. Experientia 43(4):400–401

    Article  CAS  PubMed  Google Scholar 

  15. Pauli J, Vag T, Haag R, Spieles M, Wenzel M, Kaiser WA et al (2009) An in vitro characterization study of new near infrared dyes for molecular imaging. Eur J Med Chem 44(9):3496–3503. doi:10.1016/j.ejmech.2009.01.019

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

The authors would like to thank Sabine Pohl for her excellent technical assistance and the group of Prof. Dr. Karsten Mäder and Dr. Henrike Lucas.

The project was supported by the Wilhelm-Roux-Program at the Medical Faculty of the Martin-Luther-University Halle-Wittenberg (19/38) to P.S.

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Authors and Affiliations

  1. Clinics and Policlinics of Visceral-, Transplantation-, Thoracic- and Vascular Surgery, Applied Molecular Hepatology, University of Leipzig, Liebigstraße 21, 04103, Leipzig, Germany

    Peggy Stock

  2. Department of Surgery, University of Leipzig, Leipzig, Germany

    Kristin Weber

Authors
  1. Peggy Stock
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  2. Kristin Weber
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Corresponding author

Correspondence to Peggy Stock .

Editor information

Editors and Affiliations

  1. Department of Surgery, University of Leipzig, Leipzig, Germany

    Peggy Stock

  2. Department of Surgery, University of Leipzig, Leipzig, Germany

    Bruno Christ

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Stock, P., Weber, K. (2017). Deep Digging: Far Red Imaging for the Monitoring of Transplanted Hepatocytes in Rats. In: Stock, P., Christ, B. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 1506. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6506-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6506-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6504-5

  • Online ISBN: 978-1-4939-6506-9

  • eBook Packages: Springer Protocols

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