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Immunohistochemical Analysis of Fos Protein Expression for Exploring Brain Regions Related to Central Nervous System Disorders and Drug Actions

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Book cover In Vivo Neuropharmacology and Neurophysiology

Part of the book series: Neuromethods ((NM,volume 121))

Abstract

Fos protein, an immediate early gene product, is widely used as a biological marker of neural excitation in the neuropharmacology research. Specifically, mapping analysis of Fos expression is a useful method to identify brain regions related to disease conditions (e.g., epilepsy, emotional disorders and cognitive impairments) and responses to various pathophysiological stimuli (e.g., pain, body temperature, stress, and drug treatments). Immunohistochemical staining of Fos protein can be performed by the conventional avidin-biotinylated-horseradish peroxidase complex (ABC)-diaminobenzidine (DAB) method and the counting of Fos-immunoreactivity positive neurons in the regions of interest allows topographical and quantitative analysis of neuronal network activation. In this chapter, we introduce general methods to analyze Fos protein expression, providing essential information for its application to in vivo neuropharmacology research on central nervous system (CNS) disorders and drug actions. Accurate and reliable analysis of Fos expression can help our understanding of the mechanisms underlying the pathogenesis and treatment of CNS disorders.

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Acknowledgments

This chapter was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 26460111 and 15H04892) and from the Japan Agency for Medical Research and Development (15ek0109120s0701).

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  1. Laboratory of Pharmacology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, 569-1094, Japan

    Higor A. Iha, Naofumi Kunisawa, Kentaro Tokudome, Takahiro Mukai, Masato Kinboshi, Saki Shimizu & Yukihiro Ohno

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  1. Higor A. Iha
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  2. Naofumi Kunisawa
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Correspondence to Yukihiro Ohno .

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  1. Department of Pharmacology and Toxicology, University of Innsbruck, Innsbruck, Austria

    Athineos Philippu

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Iha, H.A. et al. (2017). Immunohistochemical Analysis of Fos Protein Expression for Exploring Brain Regions Related to Central Nervous System Disorders and Drug Actions. In: Philippu, A. (eds) In Vivo Neuropharmacology and Neurophysiology. Neuromethods, vol 121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6490-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6490-1_17

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