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Neurophysiological Approaches for In Vivo Neuropharmacology

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In Vivo Neuropharmacology and Neurophysiology

Part of the book series: Neuromethods ((NM,volume 121))

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Abstract

Studies focused on the examination of the electrophysiological properties of single neurons or neural networks are most commonly performed in reduced preparations such as brain slices, disassociated neurons, or neuronal cultures. In addition, in vitro preparations are most commonly used to study the effects of neuromodulators such as monoamines, peptides, and others on the passive membrane properties, synaptic integration, and neuronal output of cells of interest. While these studies in reduced preparations are powerful for investigating mechanistic questions in identified neurons focused on drug-induced changes in ion conductances or intracellular signaling pathways, the loss of synaptic connectivity associated with these preparations limits their usefulness for solving systems neuroscience level questions or asking how pharmacodynamic drug effects act on the neuronal level when administered to intact animals. Indeed, in vivo studies have revealed that the spontaneous activity and integrative properties of neurons, and their responsiveness to neuromodulators, are largely determined by interactions between intrinsic membrane excitability and synaptic drive generated by the intact neuronal network. Given the importance of comparing outcomes from reduced preparations to those generated in the intact animal, this chapter details neurophysiological approaches for studying neuropharmacological manipulations in vivo. We focus on techniques that are used to generate information on the pharmacodynamic effects of psychotherapeutic drugs, delivered systemically as well as locally, on recordings of neuronal activity performed at the level of the single cell (e.g., single unit, juxtacellular, and intracellular recordings) and at the network level (local field potential, multi-array, amperometric, and voltammetric recordings). We also describe in detail various approaches which can be combined with the above recording techniques for local drug delivery (e.g., reverse dialysis, iontophoresis, pressure injection, microinjection, and intracellular application).

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Authors and Affiliations

  1. Department of Psychology, Franciscan University of Steubenville, Steubenville, OH, 43952, USA

    Stephen Sammut

  2. Department of Neuroscience, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL, 60064, USA

    Shreaya Chakroborty, Fernando E. Padovan-Neto & Anthony R. West Ph.D.

  3. Department of Molecular and Cellular Pharmacology, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL, 60064, USA

    J. Amiel Rosenkranz

Authors
  1. Stephen Sammut
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  2. Shreaya Chakroborty
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  3. Fernando E. Padovan-Neto
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  4. J. Amiel Rosenkranz
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  5. Anthony R. West Ph.D.
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Corresponding author

Correspondence to Anthony R. West Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Innsbruck, Innsbruck, Austria

    Athineos Philippu

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Sammut, S., Chakroborty, S., Padovan-Neto, F.E., Rosenkranz, J.A., West, A.R. (2017). Neurophysiological Approaches for In Vivo Neuropharmacology. In: Philippu, A. (eds) In Vivo Neuropharmacology and Neurophysiology. Neuromethods, vol 121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6490-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6490-1_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6488-8

  • Online ISBN: 978-1-4939-6490-1

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