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Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice

  • Rongfang Xu
  • Pengcheng Wei
  • Jianbo Yang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1498)

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) system is a newly emerging mutagenesis (gene-editing) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by Agrobacterium transformation.

Key words

CRISPR/Cas9 Rice Mutagenesis Agrobacterium transformation 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research InstituteAnhui Academy of Agricultural SciencesHefeiChina

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