Abstract
Site-directed mutagenesis has been widely used for the substitution, addition or deletion of nucleotide residues in a defined DNA sequence. QuikChange™ site-directed mutagenesis and its related protocols have been widely used for this purpose because of convenience and efficiency. We have recently demonstrated that the mechanism of the QuikChange™ site-directed mutagenesis process is different from that being proposed. The new mechanism promotes the use of partially overlapping primers and commercial PCR enzymes for efficient PCR and mutagenesis.
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Xia, Y., Xun, L. (2017). Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method. In: Reeves, A. (eds) In Vitro Mutagenesis. Methods in Molecular Biology, vol 1498. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6472-7_25
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DOI: https://doi.org/10.1007/978-1-4939-6472-7_25
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6470-3
Online ISBN: 978-1-4939-6472-7
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