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In Vitro Mutagenesis pp 163–177Cite as

Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1498))

Abstract

Dissecting the contribution of genes to virulence in fulfillment of Molecular Koch’s postulates is essential for developing prevention and treatment strategies for bacterial pathogens. This chapter will discuss the application of a targeted, intron-based insertional mutagenesis method for creating mutants in the obligate, intracellular bacterial pathogen Chlamydia trachomatis. The methods employed for intron targeting, mutant selection, and mutant verification will be outlined including available selection markers, gene targeting strategies, and potential pitfalls.

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Acknowledgments

This work was supported by NIH grants 1R21AI115238-01 and 1R15AI109566-01A1 to DJF. We thank Anna Hooppaw and Jae Claywell for reviewing our manuscript.

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Authors and Affiliations

  1. Department of Microbiology, Southern Illinois University, 1125 Lincoln Drive, Carbondale, IL, 62901, USA

    Charlotte E. Key & Derek J. Fisher

Authors
  1. Charlotte E. Key
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  2. Derek J. Fisher
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Corresponding author

Correspondence to Derek J. Fisher .

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Editors and Affiliations

  1. Coskata, Inc., Warrenville, Illinois, USA

    Andrew Reeves

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Key, C.E., Fisher, D.J. (2017). Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis . In: Reeves, A. (eds) In Vitro Mutagenesis. Methods in Molecular Biology, vol 1498. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6472-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6472-7_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6470-3

  • Online ISBN: 978-1-4939-6472-7

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