Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research
Genome-wide association (GWA) mapping is a powerful method for the identification of alleles that underlie quantitative traits. It enables one to understand how genetic variation translates into phenotypic variation. In particular, plant hormone signaling pathways play a key role in shaping phenotypes. This chapter presents a protocol for genome-wide association mapping of root traits of Arabidopsis thaliana in the context of hormone research. We describe a specific protocol for acquiring primary and lateral root trait data that is appropriate for GWA studies using FIJI (ImageJ), and subsequent GWA mapping using a user-friendly Internet application.
Key wordsNatural variation Phenotyping GWAS Hormones Root development
We are grateful to Elke Barbez, Takehiko Ogura, and Santosh Satbhai for comments and suggestion for this protocol, Ümit Seren for information about the GWA-Portal, and Matthew Watson for editing.
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