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Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research

  • Daniela Ristova
  • Wolfgang BuschEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)

Abstract

Genome-wide association (GWA) mapping is a powerful method for the identification of alleles that underlie quantitative traits. It enables one to understand how genetic variation translates into phenotypic variation. In particular, plant hormone signaling pathways play a key role in shaping phenotypes. This chapter presents a protocol for genome-wide association mapping of root traits of Arabidopsis thaliana in the context of hormone research. We describe a specific protocol for acquiring primary and lateral root trait data that is appropriate for GWA studies using FIJI (ImageJ), and subsequent GWA mapping using a user-friendly Internet application.

Key words

Natural variation Phenotyping GWAS Hormones Root development 

Notes

Acknowledgement

We are grateful to Elke Barbez, Takehiko Ogura, and Santosh Satbhai for comments and suggestion for this protocol, Ümit Seren for information about the GWA-Portal, and Matthew Watson for editing.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Gregor Mendel Institute (GMI), Austrian Academy of SciencesVienna Biocenter (VBC)ViennaAustria

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