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Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants

  • Nathan Czyzewicz
  • Elisabeth Stes
  • Ive De SmetEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)

Abstract

The first signaling peptide discovered and purified was insulin in 1921. However, it was not until 1991 that the first peptide signal, systemin, was discovered in plants. Since the discovery of systemin, peptides have emerged as a potent and diverse class of signaling molecules in plant systems. Peptides consist of small amino acid sequences, which often act as ligands of receptor kinases. However, not all peptides are created equal, and signaling peptides are grouped into several subgroups dependent on the type of post-translational processing they undergo. Here, we focus on the application of synthetic, post-translationally modified peptides (PTMPs) to plant systems, describing several methods appropriate for the use of peptides in Arabidopsis thaliana and crop models.

Key words

Post-translationally modified peptide Synthetic peptide Arabidopsis Cereal crops In vitro growth 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Nathan Czyzewicz
    • 1
  • Elisabeth Stes
    • 2
    • 3
    • 4
    • 5
  • Ive De Smet
    • 1
    • 2
    • 3
    • 6
    Email author
  1. 1.Division of Plant and Crop Sciences, School of BiosciencesUniversity of NottinghamLoughboroughUK
  2. 2.Department of Plant Systems BiologyFlemish Institute of Biotechnology (VIB)GhentBelgium
  3. 3.Department of Plant Biotechnology and BioinformaticsGhent UniversityGhentBelgium
  4. 4.Medical Biotechnology CenterFlemish Institute of Biotechnology VIBGhentBelgium
  5. 5.Department of BiochemistryGhent UniversityGhentBelgium
  6. 6.Centre for Plant Integrative BiologyUniversity of NottinghamLoughboroughUK

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