Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants

  • Nathan Czyzewicz
  • Elisabeth Stes
  • Ive De SmetEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)


The first signaling peptide discovered and purified was insulin in 1921. However, it was not until 1991 that the first peptide signal, systemin, was discovered in plants. Since the discovery of systemin, peptides have emerged as a potent and diverse class of signaling molecules in plant systems. Peptides consist of small amino acid sequences, which often act as ligands of receptor kinases. However, not all peptides are created equal, and signaling peptides are grouped into several subgroups dependent on the type of post-translational processing they undergo. Here, we focus on the application of synthetic, post-translationally modified peptides (PTMPs) to plant systems, describing several methods appropriate for the use of peptides in Arabidopsis thaliana and crop models.

Key words

Post-translationally modified peptide Synthetic peptide Arabidopsis Cereal crops In vitro growth 


  1. 1.
    Murphy E, Smith S, De Smet I (2012) Small signaling peptides in Arabidopsis development: how cells communicate over a short distance. Plant Cell 24(8):3198–3217CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Czyzewicz N, Yue K, Beeckman T et al (2013) Message in a bottle: small signalling peptide outputs during growth and development. J Exp Bot 64(17):5281–5296CrossRefPubMedGoogle Scholar
  3. 3.
    Tavormina P, De Coninck B, Nikonorova N et al (2015) The plant peptidome: an expanding repertoire of structural features and biological functions. Plant Cell 27(8):2095–2118CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Petraglia F, Florio P, Nappi C et al (2013) Peptide signaling in human placenta and membranes: autocrine, paracrine, and endocrine mechanisms. Endocr Rev 17(2):156–186Google Scholar
  5. 5.
    Reid D, Li D, Ferguson B et al (2013) Structure–function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean. J Exp Bot 64(6):1575–1585CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Shinohara H, Matsubayashi Y (2013) Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity. Plant Cell Physiol 54(3):369–374CrossRefPubMedGoogle Scholar
  7. 7.
    Czyzewicz N, Shi CL, Vu LD et al (2015) Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide. J Exp Bot 66(17):5229–5243CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Qi Z, Verma R, Gehring C et al (2010) Ca2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca2+ channels. Proc Natl Acad Sci U S A 107(49):21193–21198CrossRefPubMedPubMedCentralGoogle Scholar
  9. 10.
    Butenko M, Wildhagen M, Albert M et al (2014) Tools and strategies to match peptide-ligand receptor pairs. Plant Cell 26(5):1838–1847CrossRefPubMedPubMedCentralGoogle Scholar
  10. 11.
    Czyzewicz N, Wildhagen M, Cattaneo P et al (2015) Antagonistic peptide technology for functional dissection of CLE peptides revisited. J Exp Bot 66(17):5367–5374CrossRefPubMedPubMedCentralGoogle Scholar
  11. 13.
    Xu T, Ren S, Song X et al (2015) CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis. J Exp Bot 66(17):5217–5227CrossRefPubMedPubMedCentralGoogle Scholar
  12. 14.
    Mueller K, Chinchilla D, Albert M et al (2012) Contamination risks in work with synthetic peptides: flg22 as an example of a pirate in commercial peptide preparations. Plant Cell 24(8):3193–3197CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Nathan Czyzewicz
    • 1
  • Elisabeth Stes
    • 2
    • 3
    • 4
    • 5
  • Ive De Smet
    • 1
    • 2
    • 3
    • 6
    Email author
  1. 1.Division of Plant and Crop Sciences, School of BiosciencesUniversity of NottinghamLoughboroughUK
  2. 2.Department of Plant Systems BiologyFlemish Institute of Biotechnology (VIB)GhentBelgium
  3. 3.Department of Plant Biotechnology and BioinformaticsGhent UniversityGhentBelgium
  4. 4.Medical Biotechnology CenterFlemish Institute of Biotechnology VIBGhentBelgium
  5. 5.Department of BiochemistryGhent UniversityGhentBelgium
  6. 6.Centre for Plant Integrative BiologyUniversity of NottinghamLoughboroughUK

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