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Plant Hormones pp 259-270 | Cite as

Use of Xenopus laevis Oocytes to Study Auxin Transport

  • Astrid Fastner
  • Birgit Absmanner
  • Ulrich Z. HammesEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)

Abstract

Xenopus laevis oocytes are an expression system that is particularly well suited for the characterization of membrane transporters. Oocytes possess only very little endogenous transport systems and therefore transporters can be studied with a high signal-to-noise ratio. This book chapter provides the basic methods to use Xenopus oocytes for the characterization of transporters by radiotracer experiments. While the methods described here were established to study auxin transport they can easily be adapted to study other hormone transporters and their substrates.

Key words

Xenopus laevis oocytes Microinjection Transport Import export Radiotracer Liquid scintillation 

Notes

Acknowledgement

This work was supported by DFG grant HA3469/6-1.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Astrid Fastner
    • 1
  • Birgit Absmanner
    • 1
  • Ulrich Z. Hammes
    • 1
    Email author
  1. 1.Cell Biology and Plant BiochemistryUniversity of RegensburgRegensburgGermany

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