Grafting with Arabidopsis thaliana
Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80 % with up to 80 grafts assembled per hour can be achieved.
Key wordsArabidopsis thaliana Micro-grafting Chimeric plants Mobile molecules Graft-transmissible signal
I thank Elliot Meyerowitz and Raymond Wightman for critical reading. This work was funded by a Clare College Junior Research Fellowship and through Gatsby Charitable Trust grants GAT3272/C and GAT3273-PR1.
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