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Hormone Receptor Glycosylation

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Book cover Plant Hormones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1497))

Abstract

Glycosylation is essential for all trees of life. N-glycosylation is one of the most common covalent protein modifications and influences a large variety of cellular processes including protein folding, quality control and protein-receptor interactions. Despite recent progress in understanding of N-glycan biosynthesis, our knowledge of N-glycan function on individual plant proteins is still very limited. In this respect, plant hormone receptors are an interesting group of proteins as several of these proteins are present at distinct sites in the secretory pathway or at the plasma membrane and have numerous potential N-glycosylation sites. Identifying and characterization of N-glycan structures on these proteins is essential to investigate the functional role of this abundant protein modification. Here, a straightforward immunoblot-based approach is presented that enables the analysis of N-glycosylation on endogenous hormone receptors like the brassinosteroid receptor BRI1.

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Acknowledgements

This work was supported by a grant from the Austrian Science Fund (FWF): P23906.

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Authors and Affiliations

  1. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, BOKU, Muthgasse 18, 1190, Vienna, Austria

    Ulrike Vavra, Christiane Veit & Richard Strasser

Authors
  1. Ulrike Vavra
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  2. Christiane Veit
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  3. Richard Strasser
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Corresponding author

Correspondence to Richard Strasser .

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Editors and Affiliations

  1. IAGZ, Universität für Bodenkultur Wien, Vienna, Wien, Austria

    Jürgen Kleine-Vehn

  2. Institut für Biochemie und Biologie, Universität Potsdam, Potsdam, Germany

    Michael Sauer

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Vavra, U., Veit, C., Strasser, R. (2017). Hormone Receptor Glycosylation. In: Kleine-Vehn, J., Sauer, M. (eds) Plant Hormones. Methods in Molecular Biology, vol 1497. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6469-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6469-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6467-3

  • Online ISBN: 978-1-4939-6469-7

  • eBook Packages: Springer Protocols

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