Analyzing Cell Wall Elasticity After Hormone Treatment: An Example Using Tobacco BY-2 Cells and Auxin
Atomic force microscopy, and related nano-indentation techniques, is a valuable tool for analyzing the elastic properties of plant cell walls as they relate to changes in cell wall chemistry, changes in development, and response to hormones. Within this chapter I will describe a method for analyzing the effect of the phytohormone auxin on the cell wall elasticity of tobacco BY-2 cells. This general method may be easily altered for different experimental systems and hormones of interest.
Key wordsAtomic force microscopy Auxin Cell growth Cell wall Elasticity
I would like to thank Cris Kuhlemeier, Theres Imhof, and Alexis Peaucelle for help with initial experiments with BY-2 cells and AFM. I would also like to thank my research team at SLCU for their patience with me while writing. This work was started in the lab of C. Kuhlemeier, supported by an NSF-IRFP (Award #0853105; to S.A.B.), and completed at The Sainsbury Laboratory, University of Cambridge, supported by a Gatsby Career Development Fellowship (GAT3396/PR4) and the BBSRC (BB.L002884.1) to S.A.B.
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