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Real-Time Analysis of the Apical Hook Development

  • Qiang Zhu
  • Petra Žádníková
  • Dajo Smet
  • Dominique Van Der Straeten
  • Eva BenkováEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)

Abstract

Mechanisms for cell protection are essential for survival of multicellular organisms. In plants, the apical hook, which is transiently formed in darkness when the germinating seedling penetrates towards the soil surface, plays such protective role and shields the vitally important shoot apical meristem and cotyledons from damage. The apical hook is formed by bending of the upper hypocotyl soon after germination, and it is maintained in a closed stage while the hypocotyl continues to penetrate through the soil and rapidly opens when exposed to light in proximity of the soil surface. To uncover the complex molecular network orchestrating this spatiotemporally tightly coordinated process, monitoring of the apical hook development in real time is indispensable. Here we describe an imaging platform that enables high-resolution kinetic analysis of this dynamic developmental process.

Key words

Differential growth Apical hook development Hormonal cross talk Real-time imaging Phenotype analysis 

Notes

Acknowledgements

We thank Herman Höfte, Todor Asenov, Robert Hauschield, and Marcal Gallemi for help with the establishment of the real-time imaging platform and technical support. This work was supported by the Czech Science Foundation (GA13-39982S) to Eva Benková. Dominique Van Der Straeten acknowledges the Research Foundation Flanders for financial support (G.0656.13N). Dajo Smet holds a PhD fellowship of the Research Foundation Flanders.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Qiang Zhu
    • 1
  • Petra Žádníková
    • 2
  • Dajo Smet
    • 3
  • Dominique Van Der Straeten
    • 3
  • Eva Benková
    • 1
    Email author
  1. 1.Department of Life SciencesInstitute of Science and Technology AustriaKlosterneuburgAustria
  2. 2.Institut für GenetikHeinrich-Heine-Universität DüsseldorfDüsseldorfGermany
  3. 3.Laboratory of Functional Plant Biology, Department of PhysiologyGhent UniversityGhentBelgium

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