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Assays to Study the Fragmentation of the Golgi Complex During the G2–M Transition of the Cell Cycle

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The Golgi Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1496))

Abstract

The Golgi complex of mammalian cells is composed of stacks of flattened cisternae that are connected by tubules to form a continuous membrane system, also known as the Golgi ribbon. At the onset of mitosis, the Golgi ribbon is progressively fragmented into small tubular-vesicular clusters and it is reconstituted before completion of cytokinesis. The investigation of the mechanisms behind this reversible cycle of disassembly and reassembly has led to the identification of structural Golgi proteins and regulators. Moreover, these studies allowed to discover that disassembly of the ribbon is necessary for cell entry into mitosis. Here, we describe an in vitro assay that reproduces the mitotic Golgi fragmentation and that has been successfully employed to identify many important mechanisms and proteins involved in the mitotic Golgi reorganization.

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Acknowledgments

We apologize to those colleagues whose work we were not able to discuss due to space limitation. A.C. acknowledges the Italian Association for Cancer Research (AIRC, Milan, Italy; IG6074) for financial support.

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Authors and Affiliations

  1. Institute of Protein Biochemistry, National Research Council of Italy, Via P. Castellino 111, 80131, Naples, Italy

    Inmaculada Ayala & Antonino Colanzi

Authors
  1. Inmaculada Ayala
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  2. Antonino Colanzi
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Correspondence to Inmaculada Ayala .

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Editors and Affiliations

  1. Dept of Molecular Biology & Genetics, Cornell University, Ithaca, New York, USA

    William J. Brown

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Ayala, I., Colanzi, A. (2016). Assays to Study the Fragmentation of the Golgi Complex During the G2–M Transition of the Cell Cycle. In: Brown, W. (eds) The Golgi Complex. Methods in Molecular Biology, vol 1496. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6463-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6463-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6461-1

  • Online ISBN: 978-1-4939-6463-5

  • eBook Packages: Springer Protocols

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