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Purification Techniques for Three-Dimensional DNA Nanostructures

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Book cover 3D DNA Nanostructure

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1500))

Abstract

Separation of self-assembled three-dimensional nanostructures from excess staple strands, misfolded structures, or unattached functional elements is critical for downstream applications. Numerous purification techniques exist, with varying yields, purities, and hetero-element compatibilities. In this chapter, we focus on three such techniques—agarose gel electrophoresis, ultrafiltration, and polymeric bead pull-down—which together satisfy requirements for a range of applications.

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Author information

Authors and Affiliations

  1. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA

    Travis A. Meyer

  2. Biomolecular Nanoengineering Lab, Emory School of Medicine, Health Sciences Research Bldg E186, 1760 Haygood Drive, Atlanta, GA, 30322, USA

    Travis A. Meyer

Authors
  1. Travis A. Meyer
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Correspondence to Travis A. Meyer .

Editor information

Editors and Affiliations

  1. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Yonggang Ke

  2. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Pengfei Wang

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Meyer, T.A. (2017). Purification Techniques for Three-Dimensional DNA Nanostructures. In: Ke, Y., Wang, P. (eds) 3D DNA Nanostructure. Methods in Molecular Biology, vol 1500. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6454-3_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6454-3_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6452-9

  • Online ISBN: 978-1-4939-6454-3

  • eBook Packages: Springer Protocols

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