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Assembling RNA Nanoparticles

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3D DNA Nanostructure

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1500))

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Abstract

RNA nanoparticles are designed and self-assembled according to noncanonical interactions of naturally conserved RNA motifs and/or canonical Watson–Crick base-pairing interactions, which have potential applications in gene therapy and nanomedicine. These artificially engineered nanoparticles are mainly synthesized from in vitro transcribed RNAs, purified by denaturing and native polyacrylamide gel electrophoresis (PAGE), and characterized with native PAGE, AFM, and TEM technologies. The protocols of in vitro transcription, denaturing and native PAGE, and RNA nanoparticle self-assembly are described in detail.

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Author information

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Avenue, Nanjing, 210023, People’s Republic of China

    Shou-Jun Xiao

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  1. Shou-Jun Xiao
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Correspondence to Shou-Jun Xiao .

Editor information

Editors and Affiliations

  1. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Yonggang Ke

  2. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Pengfei Wang

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Xiao, SJ. (2017). Assembling RNA Nanoparticles. In: Ke, Y., Wang, P. (eds) 3D DNA Nanostructure. Methods in Molecular Biology, vol 1500. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6454-3_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6454-3_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6452-9

  • Online ISBN: 978-1-4939-6454-3

  • eBook Packages: Springer Protocols

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