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Spatial Organization of Enzyme Cascade on a DNA Origami Nanostructure

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3D DNA Nanostructure

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1500))

Abstract

Self-assembled DNA nanostructures hold great promise to organize multi-enzyme systems with the precise control of the geometric arrangements. Enzymes modified with single-stranded DNA anchors are assembled onto the DNA origami tiles by hybridizing with the corresponding complementary strands displayed on the surface of the DNA nanostructures. Here, we describe a protocol of assembling a two-enzyme cascade on a discrete, rectangular DNA origami tile, where the distance between enzymes is precisely controlled for investigating the distance-dependent cascade activities.

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Acknowledgement

This work is supported by an Army Research Office MURI subaward to J.F. (parent award: W911NF-12-1-0420), an Army Research Office Young Investigator award (W911NF-14-1-0434) to J.F., and a Cottrell College Science Award to J.F. Authors are grateful to Dr. Ting Zhang for proofreading the manuscript.

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Authors and Affiliations

  1. Center for Computational and Integrative Biology, Rutgers University, Camden, NJ, 08102, USA

    Jinglin Fu & Tianran Li

  2. Department of Chemistry, Rutgers University, Camden, NJ, 08102, USA

    Jinglin Fu

Authors
  1. Jinglin Fu
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  2. Tianran Li
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Corresponding author

Correspondence to Jinglin Fu .

Editor information

Editors and Affiliations

  1. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Yonggang Ke

  2. Biomolecular Nanoengineering Laboratory, Emory University, Atlanta, Georgia, USA

    Pengfei Wang

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Fu, J., Li, T. (2017). Spatial Organization of Enzyme Cascade on a DNA Origami Nanostructure. In: Ke, Y., Wang, P. (eds) 3D DNA Nanostructure. Methods in Molecular Biology, vol 1500. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6454-3_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6454-3_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6452-9

  • Online ISBN: 978-1-4939-6454-3

  • eBook Packages: Springer Protocols

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