Abstract
A small model animal Caenorhabditis elegans is particularly suitable for genetic analysis, but cell-type-specific biochemistry is a formidable task in this organism. Here we describe techniques utilizing transgenic C. elegans strains expressing epitope-tagged proteins for analyzing biochemical events, such as protein phosphorylation and formation of protein complex, in a small number of a specific group of cells at a defined stage of development. The techniques are useful for elucidating that C. elegans semaphorin-plexin signaling systems regulate epidermal morphogenesis through modulating TOR signaling and its downstream targets.
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Acknowledgements
This work was supported by JSPS KAKENHI (Grant Numbers 22370097, 25291044) and MEXT KAKENHI (Grant Number 25111708) to S.T.
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Nukazuka, A., Takagi, S. (2017). Characterizing Semaphorin Signaling In Vivo Using C. elegans . In: Terman, J. (eds) Semaphorin Signaling. Methods in Molecular Biology, vol 1493. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6448-2_34
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DOI: https://doi.org/10.1007/978-1-4939-6448-2_34
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