Abstract
Ligand-induced endocytosis of receptors exposed on the plasma membrane is a fundamental regulatory step for their functional activation and interaction with intracellular signal transducers. Thus, elucidating the timing of endocytosis and tracing the intracellular fate of receptors is instrumental to understand their signaling cascade in different conditions. Here we describe an assay for the study of endocytosis and intracellular trafficking of individual surface receptors, in living cells subject to different experimental challenges. We applied this method for studying the functional interaction between semaphorin coreceptor Neuropilin-1 and a tyrosine kinase receptor exposed on the cell surface.
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Acknowledgments
The reported research activity in the authors’ lab is supported by the Italian Association for Cancer Research (AIRC-IG #2014-15179) and the Fondazione Piemontese per la Ricerca sul Cancro (FPRC-ONLUS) Grant “MIUR 2010 Vaschetto—5 per mille 2010 MIUR”.
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Rizzolio, S., Tamagnone, L. (2017). Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors. In: Terman, J. (eds) Semaphorin Signaling. Methods in Molecular Biology, vol 1493. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6448-2_23
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DOI: https://doi.org/10.1007/978-1-4939-6448-2_23
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