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Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse

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Semaphorin Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1493))

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Abstract

Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

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Acknowledgments

This work was supported by NIH grant; contract grant number: NS026150.

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Authors and Affiliations

  1. Department of Neuroscience, Washington University School of Medicine, Box 8108, 660 S Euclid, Saint Louis, MO, 63110, USA

    Paul C. Bridgman

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  1. Paul C. Bridgman
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Correspondence to Paul C. Bridgman .

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Editors and Affiliations

  1. Departments of Neuroscience and Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Jonathan R. Terman

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Bridgman, P.C. (2017). Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse. In: Terman, J. (eds) Semaphorin Signaling. Methods in Molecular Biology, vol 1493. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6448-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6448-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6446-8

  • Online ISBN: 978-1-4939-6448-2

  • eBook Packages: Springer Protocols

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