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Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells

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Activity-Based Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1491))

Abstract

Posttranslational modification of proteins with the small ubiquitin-like modifier (SUMO) regulates protein function in the context of cell cycle and DNA repair. The occurrence of SUMOylation is less frequent as compared to protein modification with ubiquitin, and appears to be controlled by a smaller pool of conjugating and deconjugating enzymes. Mass spectrometry has been instrumental in defining specific as well as proteome-wide views of SUMO-dependent biological processes, and several methodological approaches have been developed in the recent past. Here, we provide an overview of the latest experimental approaches to the study of SUMOylation, and also describe hands-on protocols using a combination of biochemistry and mass spectrometry-based technologies to profile proteins that are SUMOylated in human cells.

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Acknowledgments

We thank members of the Kessler, Hickson and Liu groups for helpful discussions, and Nicola Ternette and Roman Fischer for help with the analysis by mass spectrometry. This work was supported by CRUK (B.M.K.), John Fell Fund 133/075 (B.M.K.), and the Wellcome Trust 097813/Z/11/Z (B.M.K.); the Danish National Research Foundation (DNRF) (I.D.H., Y.L.), the Danish Medical Research council (I.D.H.), and the European Research Council (I.D.H.). S.B. was funded by a Ph.D. fellowship from the Faculty of Health and Medical Sciences, University of Copenhagen.

Conflicts of Interest

B.M.K. is associated with Cancer Research Technologies and Forma Therapeutics.

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Author notes

  1. Joanna F. McGouran

    Present address: School of Chemistry, Trinity College Dublin, University of Dublin, College Green, Dublin 2, Ireland

Authors and Affiliations

  1. Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK

    Benedikt M. Kessler & Joanna F. McGouran

  2. Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Panum Institute, Astra Zeneca, Godsmottagningen MA1, Pepparedsleden, 43183, Mölndal, Sweden

    Sara Bursomanno, Ian D. Hickson & Ying Liu

Authors
  1. Benedikt M. Kessler
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  2. Sara Bursomanno
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  3. Joanna F. McGouran
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  4. Ian D. Hickson
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  5. Ying Liu
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Corresponding author

Correspondence to Benedikt M. Kessler .

Editor information

Editors and Affiliations

  1. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Herman S. Overkleeft

  2. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Bogdan I. Florea

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Kessler, B.M., Bursomanno, S., McGouran, J.F., Hickson, I.D., Liu, Y. (2017). Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. In: Overkleeft, H., Florea, B. (eds) Activity-Based Proteomics. Methods in Molecular Biology, vol 1491. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6439-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6439-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6437-6

  • Online ISBN: 978-1-4939-6439-0

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