Abstract
Brevibacillus choshinensis is an excellent host for the production of secretory proteins. This host has also been applied successfully to efficient production of CCN proteins. Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with Brevibacillus as a host, cultivation methods for protein production, and methods of purification for domain proteins using his-tag.
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References
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Hanagata, H., Mizukami, M. (2017). Production of Recombinant CCN Proteins by Brevibacillus choshinensis . In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_9
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DOI: https://doi.org/10.1007/978-1-4939-6430-7_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6428-4
Online ISBN: 978-1-4939-6430-7
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