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CCN Proteins pp 283–308Cite as

Gene Expression Analysis of CCN Protein in Bone Under Mechanical Stress

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1489))

Abstract

To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specific phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fluid shear stress and substrate strain in vitro, and compression or tension force in vivo.

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Acknowledgement

This work was supported by grants to T.T.-Y., T.F. and N.T. from Ministry of Education, Culture, Sports, Science and Technology in Japan, Japan Society for the Promotion of Science, and Japan Science and Technology Agency.

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Authors and Affiliations

  1. Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan

    Teruko Takano-Yamamoto D.D.S., Ph.D., Tomohiro Fukunaga & Nobuo Takeshita

Authors
  1. Teruko Takano-Yamamoto D.D.S., Ph.D.
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  2. Tomohiro Fukunaga
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  3. Nobuo Takeshita
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Corresponding author

Correspondence to Teruko Takano-Yamamoto D.D.S., Ph.D. .

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Editors and Affiliations

  1. Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Masaharu Takigawa

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Takano-Yamamoto, T., Fukunaga, T., Takeshita, N. (2017). Gene Expression Analysis of CCN Protein in Bone Under Mechanical Stress. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_26

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  • DOI: https://doi.org/10.1007/978-1-4939-6430-7_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6428-4

  • Online ISBN: 978-1-4939-6430-7

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