Abstract
Function of CCN family proteins is determined by the interactions with multiple cofactors that are present in microenvironment. Therefore, finding out these cofactors is critically important in understanding the molecular function of the CCN family members. For this objective, bacteriophage random peptide display library is a quite feasible tool. In this library, each filamentous bacteriophage is designed to display an oligopeptide of random 12–16 amino acid residues on its surface. Bacteriophage clones that possess the peptides that bind to a CCN family protein are selected through several cycles of a process designated biopanning or affinity selection. By determining the nucleotide sequence of the DNA that encodes the displayed peptide, oligopeptides that specifically bind to the CCN family member can be specified. Obtained peptide sequences can be utilized for designing peptide aptamers for the CCN family protein, or as a key sequence to find out new CCN family cofactor candidates in silico.
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Acknowledgement
We gratefully thank Ms. Yoshiko Miyake for secretarial assistance. Funding: This study was supported by grants from the program Grants-in-aid for Scientific Research (B) [JP15H05014] to M.T. and (C) [JP25462886] to S.K. from the Japan Society for the Promotion of Science and from Wesco Scientific Promotion Foundation.
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Kubota, S., Kawaki, H., Takigawa, M. (2017). Protocols for Screening Peptide Motifs Binding to CCN Family Proteins. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_16
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DOI: https://doi.org/10.1007/978-1-4939-6430-7_16
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