ERK Signaling

Volume 1487 of the series Methods in Molecular Biology pp 317-335


Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development

  • Jessica Jie ChenAffiliated withThe University of Texas Graduate School of Biomedical Sciences
  • , Swathi ArurAffiliated withThe University of Texas Graduate School of Biomedical SciencesDepartment of Genetics, The University of Texas MD Anderson Cancer Center Email author 

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The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signaling has been shown to regulate over seven distinct biological processes in a temporal and sequential manner. To fully elucidate how ERK signaling cascade orchestrates these different biological processes in vivo, identification of the direct functional substrates of the pathway is critical. This chapter describes the methods that were used to identify ERK substrates in a global manner and study their functions in the germline. These approaches can also be generally applied to study ERK-dependent biological processes in other systems.

Key words

C. elegans oogenesis ERK substrates Functional genomics