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Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development

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ERK Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1487))

Abstract

The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signaling has been shown to regulate over seven distinct biological processes in a temporal and sequential manner. To fully elucidate how ERK signaling cascade orchestrates these different biological processes in vivo, identification of the direct functional substrates of the pathway is critical. This chapter describes the methods that were used to identify ERK substrates in a global manner and study their functions in the germline. These approaches can also be generally applied to study ERK-dependent biological processes in other systems.

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Acknowledgements

J.J.C. is funded by the UT Graduate School of Biomedical Sciences Dean’s Scholarship. NIH RO1 GM 98200, American Cancer Society Grant ACS RSG014-044-DDC and CPRIT RP160023 grants fund work in the Arur Lab.

Author information

Authors and Affiliations

  1. The University of Texas Graduate School of Biomedical Sciences, Houston, TX, USA

    Jessica Jie Chen & Swathi Arur Ph.D.

  2. Department of Genetics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX, 77030, USA

    Swathi Arur Ph.D.

Authors
  1. Jessica Jie Chen
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  2. Swathi Arur Ph.D.
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Corresponding author

Correspondence to Swathi Arur Ph.D. .

Editor information

Editors and Affiliations

  1. Institut de Biologia Molecular de Barcelona-CSIC, Parc Científic de Barcelona, Barcelona, Spain

    Gerardo Jimenez

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Chen, J.J., Arur, S. (2017). Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development. In: Jimenez, G. (eds) ERK Signaling. Methods in Molecular Biology, vol 1487. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6424-6_24

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  • DOI: https://doi.org/10.1007/978-1-4939-6424-6_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6422-2

  • Online ISBN: 978-1-4939-6424-6

  • eBook Packages: Springer Protocols

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