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Storage and Lyophilization of Pure Proteins

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Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1485))

Abstract

This article outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation, and the use of various types of stabilizing additives. It sets out the principles of lyophilization (otherwise known as freeze-drying, a complex process comprising freezing, primary dying, and secondary drying stages). There follows a general procedure for the use of lyophilizer apparatus with emphasis on best practice and on pitfalls to avoid. The use of modulated differential scanning calorimetry to measure the glass transition temperature, a key parameter in the design and successful operation of lyophilization processes, is described.

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Authors and Affiliations

  1. School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland

    Ciarán Ó’Fágáin

  2. National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland

    Ciarán Ó’Fágáin

  3. Pfizer Ireland Pharmaceuticals, Grange Castle Business Park, Clondalkin, Dublin 22, Ireland

    Keith Colliton

Authors
  1. Ciarán Ó’Fágáin
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  2. Keith Colliton
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Correspondence to Ciarán Ó’Fágáin .

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Editors and Affiliations

  1. School of Biotechnology, Dublin City University, Dublin, Ireland

    Dermot Walls

  2. Department of Applied Sciences, Dundalk Institute of Technology, Dublin, Ireland

    Sinéad T. Loughran

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Ó’Fágáin, C., Colliton, K. (2017). Storage and Lyophilization of Pure Proteins. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 1485. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6412-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6412-3_9

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  • Online ISBN: 978-1-4939-6412-3

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