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Protein Quantitation and Analysis of Purity

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Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1485))

Abstract

The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical applications. The large number and variety of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on such factors as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-Polymerase Chain Reaction.

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Author information

Authors and Affiliations

  1. Department of Life Sciences, Institute of Technology Sligo, Ash Lane, Sligo, Ireland

    Eva M. Campion

  2. Department of Applied Sciences, Dundalk Institute of Technology, Dundalk, Ireland

    Sinéad T. Loughran

  3. School of Biotechnology, Dublin City University, Dublin 9, Ireland

    Dermot Walls

  4. National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland

    Dermot Walls

Authors
  1. Eva M. Campion
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  2. Sinéad T. Loughran
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  3. Dermot Walls
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Corresponding author

Correspondence to Eva M. Campion .

Editor information

Editors and Affiliations

  1. School of Biotechnology, Dublin City University, Dublin, Ireland

    Dermot Walls

  2. Department of Applied Sciences, Dundalk Institute of Technology, Dublin, Ireland

    Sinéad T. Loughran

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Campion, E.M., Loughran, S.T., Walls, D. (2017). Protein Quantitation and Analysis of Purity. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 1485. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6412-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6412-3_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6410-9

  • Online ISBN: 978-1-4939-6412-3

  • eBook Packages: Springer Protocols

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