Abstract
The hyphenation of capillary electrophoresis and electrospray ionization–mass spectrometry is a powerful tool for peptide and protein analysis. It provides high separation power in combination with sensitive and selective detection and the possibility of analyte identification. Unfortunately, many proven capillary electrophoresis methods are not compatible with electrospray-mass spectrometry since several compounds of best separating background electrolytes are interfering in the electrospray ionization. Here, we describe a two-dimensional capillary electrophoresis system using the second dimension as a cleanup stage in order to remove interfering compounds to enable electrospray–mass spectrometry coupling.
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Kohl, F.J., Neusüß, C. (2016). CZE–CZE ESI–MS Coupling with a Fully Isolated Mechanical Valve. In: Schmitt-Kopplin, P. (eds) Capillary Electrophoresis. Methods in Molecular Biology, vol 1483. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6403-1_9
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DOI: https://doi.org/10.1007/978-1-4939-6403-1_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6401-7
Online ISBN: 978-1-4939-6403-1
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