Abstract
Capillary electrophoresis appeared to be a powerful and reliable technique to analyze the diversity of wine compounds. Wine presents a great variety of natural chemicals coming from the grape berry extraction and the fermentation processes. The first and more abundant after water, ethanol has been quantified in wines via capillary electrophoresis. Other families like organic acids, neutral and acid sugars, polyphenols, amines, thiols, vitamins, and soluble proteins are electrophoretically separated from the complex matrix.
Here, we will focus on the different methodologies that have been employed to conduct properly capillary electrophoresis in wine analysis.
Two examples informing on wine chemistry obtained by capillary electrophoresis will be detailed. They concern polyphenol analysis and protein profiling. The first category is a well-developed quantitative approach important for the quality and the antioxidant properties conferred to wine. The second aspect involves more research aspects dealing with microbiota infections in the vineyard or in the grape as well as enological practices.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jorgenson JW, Lukacs KD (1981) Zone electrophoresis in open-tubular glass capillaries. Anal Chem 53:1298–1302
VanOrman BB, Liversidge GG, McIntire GL, Olefirowicz TM, Ewing AG (1990) Effects of buffer composition on electroosmotic flow in capillary electrophoresis. J Microcolumn Sep 2:176–180
Gu X, Creasy L, Kester A, Zeece M (1999) Capillary electrophoretic determination of resveratrol in wines. J Agric Food Chem 47:3223–3227
Noe CR, Lachmann B, Möllenbeck S, Richter P (1999) Determination of reducing sugars in selected beverages by capillary electrophoresis. Z Lebensm Unters Forsch 208:148–152
Huang X, Luckey JA, Gordon MJ, Zare RN (1989) Quantitative determination of low molecular weight carboxylic acids by capillary zone electrophoresis/conductivity detection. Anal Chem 61:766–770
Collins TS, Miller CA, Altria KD, Waterhouse AL (1997) Development of a rapid method for the analysis of ethanol in wines using capillary electrophoresis. Am J Enol Vitic 48:280–284
Nouadje G, Siméon N, Dedieu F, Nertz M, Puig P, Couderc F (1997) Determination of twenty eight biogenic amines and amino acids during wine aging by micellar electrokinetic chromatography and laser-induced fluorescence detection. J Chromatogr A 765:337–343
Kovács Á, Simon-Sarkadi L, Ganzler K (1999) Determination of biogenic amines by capillary electrophoresis. J Chromatogr A 836:305–313
Rodríguez-Delgado M, Malovaná S, Montelongo F, Cifuentes A (2002) Fast analysis of proteins in wines by capillary gel electrophoresis. Eur Food Res Technol 214:536–540
Hsieh M-C, Lin C-H (2004) On-line identification of trans-resveratrol in red wine using a sweeping technique combined with capillary electrophoresis/77 K fluorescence spectroscopy. Electrophoresis 25:677–682
de Valme García Moreno M, Castro Mejías R, Natera Marín R, García Barroso C (2002) Analysis of sugar acids by capillary electrophoresis with indirect UV detection. Application to samples of must and wine. Eur Food Res Technol 215:255–259
Peres RG, Moraes EP, Micke GA, Tonin FG, Tavares MFM, Rodriguez-Amaya DB (2009) Rapid method for the determination of organic acids in wine by capillary electrophoresis with indirect UV detection. Food Control 20:548–552
Qin W, Wei H, Li SFY (2003) 1,3-Dialkylimidazolium-based room-temperature ionic liquids as background electrolyte and coating material in aqueous capillary electrophoresis. J Chromatogr A 985:447–454
Minussi RC, Rossi M, Bologna L, Cordi LV, Rotilio D, Pastore GM, Durán N (2003) Phenolic compounds and total antioxidant potential of commercial wines. Food Chem 82:409–416
Lavigne V, Pons A, Dubourdieu D (2007) Assay of glutathione in must and wines using capillary electrophoresis and laser-induced fluorescence detection: changes in concentration in dry white wines during alcoholic fermentation and aging. J Chromatogr A 1139:130–135
Vanhoenacker G, De Villiers A, Lazou K, De Keukeleire D, Sandra P (2001) Comparison of high-performance liquid chromatography—mass spectroscopy and capillary electrophoresis—mass spectroscopy for the analysis of phenolic compounds in diethyl ether extracts of red wines. Chromatographia 54:309–315
Simó C, Moreno-Arribas MV, Cifuentes A (2008) Ion-trap versus time-of-flight mass spectrometry coupled to capillary electrophoresis to analyze biogenic amines in wine. J Chromatogr A 1195:150–156
Santos B, Simonet BM, Ríos A, Valcárcel M (2004) Direct automatic determination of biogenic amines in wine by flow injection-capillary electrophoresis-mass spectrometry. Electrophoresis 25:3427–3433
Simó C, Elvira C, González N, San Román J, Barbas C, Cifuentes A (2004) Capillary electrophoresis-mass spectrometry of basic proteins using a new physically adsorbed polymer coating. Some applications in food analysis. Electrophoresis 25:2056–2064
Zhao SS, Zhong X, Tie C, Chen DD (2012) Capillary electrophoresis-mass spectrometry for analysis of complex samples. Proteomics 12:2991–3012
Wang S-P, Huang K-J (2004) Determination of flavonoids by high-performance liquid chromatography and capillary electrophoresis. J Chromatogr A 1032:273–279
Gu X, Chu Q, O’Dwyer M, Zeece M (2000) Analysis of resveratrol in wine by capillary electrophoresis. J Chromatogr A 881:471–481
Brandolini V, Maietti A, Tedeschi P, Durini E, Vertuani S, Manfredini S (2002) Capillary electrophoresis determination, synthesis, and stability of resveratrol and related 3-O-β-d-glucopyranosides. J Agric Food Chem 50:7407–7411
Panossian A, Mamikonyan G, Torosyan M, Gabrielyan E, Mkhitaryan S (2001) Analysis of aromatic aldehydes in brandy and wine by high-performance capillary electrophoresis. Anal Chem 73:4379–4383
Luguera C, Moreno-Arribas V, Pueyo E, Polo MC (1997) Capillary electrophoretic analysis of wine proteins. Modifications during the manufacture of sparkling wines. J Agric Food Chem 45:3766–3770
Luguera C, Moreno-Arribas V, Pueyo E, Bartolome B, Polo MC (1998) Fractionation and partial characterization of protein fractions present at different stages of the production of sparkling wines. Food Chem 63:465–471
Dizy M, Bisson LF (1999) White wine protein analysis by capillary zone electrophoresis. Am J Enol Vitic 50:120–127
Waterhouse AL (2002) Wine phenolics. Ann N Y Acad Sci 957:21–36
Herrero-Martínez JM, Sanmartin M, Rosés M, Bosch E, Ràfols C (2005) Determination of dissociation constants of flavonoids by capillary electrophoresis. Electrophoresis 26:1886–1895
Roullier-Gall C, Boutegrabet L, Gougeon RD, Schmitt-Kopplin P (2014) A grape and wine chemodiversity comparison of different appellations in Burgundy: vintage vs terroir effects. Food Chem 152:100–107
He Y, Harir M, Chen G, Gougeon RD, Zhang L, Huang X, Schmitt-Kopplin P (2014) Capillary electrokinetic fractionation mass spectrometry (CEkF/MS): technology setup and application to metabolite fractionation from complex samples coupled at-line with ultrahigh-resolution mass spectrometry. Electrophoresis 35:1965–1975
Schmalzing D, Piggee CA, Foret F, Carrilho E, Karger BL (1993) Characterization and performance of a neutral hydrophilic coating for the capillary electrophoretic separation of biopolymers. J Chromatogr A 652:149–159
Dorrestein E, Ferreira RB, Laureano O, Teixeira AR (1995) Electrophoretic and FPLC analysis of soluble proteins in four portuguese wines. Am J Enol Vitic 46:235–242
Cilindre C, Jégou S, Hovasse A, Schaeffer C, Castro AJ, Clément C, Van Dorsselaer A, Jeandet P, Marchal R (2008) Proteomic approach to identify champagne wine proteins as modified by Botrytis cinerea infection. J Proteome Res 7:1199–1208
Cataldi TRI, Nardiello D, Scrano L, Scopa A (2002) Assay of riboflavin in sample wines by capillary zone electrophoresis and laser-induced fluorescence detection. J Agric Food Chem 50:6643–6647
Cataldi TRI, Nardiello D, De Benedetto GE, Bufo SA (2002) Optimizing separation conditions for riboflavin, flavin mononucleotide and flavin adenine dinucleotide in capillary zone electrophoresis with laser-induced fluorescence detection. J Chromatogr A 968:229–239
Saavedra L, Barbas C (2003) Validated capillary electrophoresis method for small-anions measurement in wines. Electrophoresis 24:2235–2243
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Coelho, C., Bagala, F., Gougeon, R.D., Schmitt-Kopplin, P. (2016). Capillary Electrophoresis in Wine Science. In: Schmitt-Kopplin, P. (eds) Capillary Electrophoresis. Methods in Molecular Biology, vol 1483. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6403-1_23
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6403-1_23
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6401-7
Online ISBN: 978-1-4939-6403-1
eBook Packages: Springer Protocols