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Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry

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Capillary Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1483))

Abstract

During the recent years, capillary electrophoresis (CE) has been fully established as a powerful tool in separation sciences as well as in element speciation. This road of success is based on the rapid analysis time, low sample requirements, high separation efficiency, and low operating costs of CE. Inductively coupled plasma mass spectrometry (ICP-MS) is known for superior detection and multielement capability. Consequently, the combination of both instruments is approved for analysis of complex sample types at low element concentrations which require high detection power. Also the diversity of potential applications brings CE–ICP-MS coupling into central focus of element speciation. The key to successful combination of ICP-MS as an (multi-)element selective detector for CE is the availability of a suitable and effective interface.

Therefore, this chapter summarizes the most important and basic principles about coupling of capillary electrophoresis to ICP-MS. Specifically, the major requirements for interfacing are described and technical solutions are given. Such solutions include the closing of the electrical circuit from CE at the nebulization, the adoption of flow rates for efficient nebulization, the reduction of a suction flow through the capillary, caused by the nebulizer, and maintaining the high separation resolution from CE across the interface for ICP-MS detection. Additionally, detailed information is presented to determine and quantify the siphoning suction through the CE capillary by the nebulizer. Finally, two applications, namely, the manganese and selenium speciation in cerebrospinal fluid are shown as examples, providing the relevant operational parameter.

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Abbreviations

CE:

Capillary electrophoresis

CZE:

Free zone electrophoresis

ICP-MS:

Inductively coupled plasma mass spectrometry

IEF:

Isoelectric focusing

ITP:

Isotachophoresis

LC:

Liquid chromatography

LoD:

Limit of detection

MEKC:

Micellar electrokinetic chromatography

RF:

Radiofrequency

USN:

Ultrasonic nebulizer

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Authors and Affiliations

  1. Research Unit Analytical BioGeoChemistry, Helmholtz Center Munich—German Research Center for Environmental Health GmbH, Ingolstädter Landstr. 1, Neuherberg, D-85764, Germany

    Bernhard Michalke

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  1. Bernhard Michalke
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Correspondence to Bernhard Michalke .

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Editors and Affiliations

  1. Helmholtz Zentrum Munchen, Neuherberg, Germany

    Philippe Schmitt-Kopplin

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Michalke, B. (2016). Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry. In: Schmitt-Kopplin, P. (eds) Capillary Electrophoresis. Methods in Molecular Biology, vol 1483. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6403-1_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6403-1_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6401-7

  • Online ISBN: 978-1-4939-6403-1

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