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Delivery of the Porcupine Inhibitor WNT974 in Mice

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Wnt Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1481))

Abstract

We describe here a technique for delivering the porcupine inhibitor WNT974 (formerly LGK974) in mice. The protocol entails once-a-day oral delivery of WNT974 for up to 3 months at a concentration sufficient to achieve systemic Wnt pathway inhibition with limited toxicity as measured by weight change. This route of delivery enables extended durations of Wnt signaling inhibition in a mammalian model organism.

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6393-5_18

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Acknowledgement

This work was supported by the NIH (R01 CA168761, R01CA196851), CPRIT (RP130212), and the Welch Foundation (I-1665).

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Authors and Affiliations

  1. Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9039, USA

    Li-shu Zhang & Lawrence Lum

Authors
  1. Li-shu Zhang
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  2. Lawrence Lum
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Editor information

Editors and Affiliations

  1. University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Quinn Barrett

  2. Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Lawrence Lum

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Zhang, Ls., Lum, L. (2016). Delivery of the Porcupine Inhibitor WNT974 in Mice. In: Barrett, Q., Lum, L. (eds) Wnt Signaling. Methods in Molecular Biology, vol 1481. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6393-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6393-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6391-1

  • Online ISBN: 978-1-4939-6393-5

  • eBook Packages: Springer Protocols

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