Wnt Signaling pp 111-117 | Cite as

Delivery of the Porcupine Inhibitor WNT974 in Mice

  • Li-shu Zhang
  • Lawrence Lum
Part of the Methods in Molecular Biology book series (MIMB, volume 1481)


We describe here a technique for delivering the porcupine inhibitor WNT974 (formerly LGK974) in mice. The protocol entails once-a-day oral delivery of WNT974 for up to 3 months at a concentration sufficient to achieve systemic Wnt pathway inhibition with limited toxicity as measured by weight change. This route of delivery enables extended durations of Wnt signaling inhibition in a mammalian model organism.

Key words

Porcupine Wnt974/LGK974 Mice Drug delivery 



This work was supported by the NIH (R01 CA168761, R01CA196851), CPRIT (RP130212), and the Welch Foundation (I-1665).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Li-shu Zhang
    • 1
  • Lawrence Lum
    • 1
  1. 1.Department of Cell BiologyUniversity of Texas Southwestern Medical CenterDallasUSA

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