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Noncoding RNA Interplay with the Genome

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Polycomb Group Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1480))

Abstract

The majority of our genome is transcribed to produce RNA molecules that are mostly noncoding. Among them, long noncoding RNAs (lncRNAs) are the most numerous and functionally versatile class.

LncRNAs have emerged as key regulators of gene expression at multiple levels.

This section describes bioinformatics aspects important for lncRNA discovery and molecular approaches to perform structure-function characterization of this exciting class of regulatory molecules.

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References

  1. Higgs PG, Lehman N (2014) The RNA World: molecular cooperation at the origins of life. Nat Rev Genet 16:7–17

    Article  PubMed  Google Scholar 

  2. Kapranov P et al (2005) Examples of the complex architecture of the human transcriptome revealed by RACE and high-density tiling arrays. Genome Res 15:987–997

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Carninci P et al (2005) The transcriptional landscape of the mammalian genome. Science 309:1559–1563

    Article  CAS  PubMed  Google Scholar 

  4. Taft RJ, Pheasant M, Mattick JS (2007) The relationship between non-protein-coding DNA and eukaryotic complexity. Bioessays 29:288–299

    Article  CAS  PubMed  Google Scholar 

  5. Mallory AC, Shkumatava A (2015) LncRNAs in vertebrates: advances and challenges. Biochimie 117:3–14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Mattick JS, Rinn JL (2015) Discovery and annotation of long noncoding RNAs. Nat Struct Mol Biol 22:5–7

    Article  CAS  PubMed  Google Scholar 

  7. Clark MB et al (2015) Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing. Nat Methods 12:339–342

    Article  CAS  PubMed  Google Scholar 

  8. Gloss BS, Dinger ME (2015) The specificity of long noncoding RNA expression. Biochim Biophys Acta. doi:10.1016/j.bbagrm.2015.08.005

    PubMed  Google Scholar 

  9. Chu C, Spitale RC, Chang HY (2015) Technologies to probe functions and mechanisms of long noncoding RNAs. Nat Struct Mol Biol 22:29–35

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Davide Gabellini .

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Gabellini, D. (2016). Noncoding RNA Interplay with the Genome. In: Lanzuolo, C., Bodega, B. (eds) Polycomb Group Proteins. Methods in Molecular Biology, vol 1480. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6380-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6380-5_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6378-2

  • Online ISBN: 978-1-4939-6380-5

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