Abstract
This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq).
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6380-5_28
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6380-5_28
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References
Gilmour DS, Lis JT (1985) In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster. Mol Cell Biol 5(8):2009–2018
O’Neill LP, Turner BM (2003) Immunoprecipitation of native chromatin: NChIP. Methods 31(1):76–82
Li XY et al. (2014) Establishment of regions of genomic activity during the Drosophila maternal to zygotic transition. Elife 3
Lott SE et al (2011) Noncanonical compensation of zygotic X transcription in early Drosophila melanogaster development revealed through single-embryo RNA-seq. PLoS Biol 9(2), e1000590
Hartenstein V, Camposortega JA (1985) Fate-mapping in wild-type drosophila-melanogaster 1. The spatio-temporal pattern of embryonic-cell divisions. Wilhelm Rouxs Arch Dev Biol 194(4):181–195
Gavrilov A, Razin SV, Cavalli G (2015) In vivo formaldehyde cross-linking: it is time for black box analysis. Brief Funct Genomics 14(2):163–165
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Löser, E., Latreille, D., Iovino, N. (2016). Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos. In: Lanzuolo, C., Bodega, B. (eds) Polycomb Group Proteins. Methods in Molecular Biology, vol 1480. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6380-5_3
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DOI: https://doi.org/10.1007/978-1-4939-6380-5_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6378-2
Online ISBN: 978-1-4939-6380-5
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