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Drosophila pp 215–226Cite as

Cultivation and Live Imaging of Drosophila Ovaries

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1478))

Abstract

Drosophila egg chamber development depends on a number of dynamic cellular processes that contribute to the final shape and function of the egg. We can gain insight into the mechanisms underlying these events by combining the power of Drosophila genetics and ex vivo live imaging. During developmental stages 1–8, egg chambers rotate around their anterior-posterior axes due to collective migration of the follicular epithelium. This motion is required for the proper elongation of the egg chamber. Here, we describe how to prepare stage 1–8 egg chambers for live imaging. We provide alternate protocols for the use of inverted or upright microscopes and describe ways to stabilize egg chambers to reduce drift during imaging. We discuss the advantages and limitations of these methods to assist the researcher in choosing an appropriate method based on experimental need and available resources.

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Acknowledgements

We thank members of the Horne-Badovinac lab for input, Guillermina Ramirez-San Juan for the dissection video, and Claire Stevenson for the images in Fig. 1c. M.C. was supported by NIH T32 GM007183 and work in the Horne-Badovinac lab is supported by NIH R01 GM094276.

Author information

Authors and Affiliations

  1. Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA

    Maureen Cetera & Sally Horne-Badovinac

  2. Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA

    Maureen Cetera & Sally Horne-Badovinac

  3. Department of Biological Sciences, Butler University, 4600 Sunset Boulevard, Indianapolis, IN, 46208, USA

    Lindsay Lewellyn

Authors
  1. Maureen Cetera
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  2. Lindsay Lewellyn
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  3. Sally Horne-Badovinac
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Corresponding author

Correspondence to Sally Horne-Badovinac .

Editor information

Editors and Affiliations

  1. Institute of Genetics, Technische Universität Dresden, Dresden, Germany

    Christian Dahmann

1 Electronic supplementary material

Drosophila ovary dissection. Video showing dissection of Drosophila ovaries using a stereomicroscope. Alternate dissection methods are shown for acquiring stage 6-8 or stage 1-5 egg chambers. After dissection, healthy ovarioles are sorted and older egg chambers are trimmed away. Please see Fig. 2 for stills of this video and a detailed procedural description.

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Cetera, M., Lewellyn, L., Horne-Badovinac, S. (2016). Cultivation and Live Imaging of Drosophila Ovaries. In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 1478. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6371-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6371-3_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6369-0

  • Online ISBN: 978-1-4939-6371-3

  • eBook Packages: Springer Protocols

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