Abstract
The diameter and sphericity of alginate-poly-l-lysine-alginate microcapsules, determined by the size and the shape of calcium alginate microspheres, affect their in vivo durability and biocompatibility and the results of transplantation. The commonly used air-jet spray method generates microspheres with a wider variation in diameter, larger sphere morphology, and evenly distributed encapsulated cells. In order to overcome these drawbacks, we designed a field effect microparticle generator to create a stable electric field to prepare microparticles with a smaller diameter and more uniform morphology. Using this electric field microparticle generator the encapsulated cells will be located at the periphery of the microspheres, and thus the supply of oxygen and nutrients for the encapsulated cells will be improved compared with the centrally located encapsulated cells in the air-jet spray method.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lim F, Sun AM (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210:908–910
Sun AM, Vacek I, Sun YL, Ma X, Zhou D (1992) In vitro and in vivo evaluation of microencapsulated porcine islets. ASAIO J 38:125–127
Soon-Shiong P, Feldman E, Nelson R, Komtebedde J, Smidsrod O, Skjak-Braek G, Espevik T, Heintz R, Lee M (1992) Successful reversal of spontaneous diabetes in dogs by intraperitoneal microencapsulated islets. Transplantation 54:769–774
Soon-Shiong P, Heintz RE, Merideth N, Yao QX, Yao Z, Zheng T, Murphy M, Moloney MK, Schmehl M, Harris M, Mendez R, Sandford PA (1994) Insulin independence in a type 1 diabetic patient after encapsulated islet transplantation. Lancet 343:950–951
Fu XW, Sun AM (1989) Microencapsulated parathyroid cells as a bioartificial parathyroid. Transplantation 47:432–435
Cai Z, Shi ZQ, Sherman M, Sun AM (1989) Development and evaluation of a system of microencapsulation of primary rat hepatocytes. Hepatology 10:855–860
Sun AM, Cai Z, Shi Z, Ma F, O’Shea GM (1987) Microencapsulated hepatocytes: an in vitro and in vivo study. Biomater Artif Cells Artif Organs 15:483–496
Zhang Y, Chen XM, Sun DL (2014) Effects of coencapsulation of hepatocytes with adipose-derived stem cells in the treatment of rats with acute-on-chronic liver failure. Int J Artif Organs 37:133–141
Aebischer P, Tresco PA, Sagen J, Winn SR (1991) Transplantation of microencapsulated bovine chromattin cells reduces lesion-induced rotational asymmetry in rats. Brain Res 560:43–49
Goren A, Gilert A, Meyron-Holtz E, Melamed D, Machluf M (2012) Alginate encapsulated cells secreting Fas-ligand reduce lymphoma carcinogenicity. Cancer Sci 103:116–124
Rodes L, Tomaro-Duchesneau C, Saha S, Paul A, Malhotra M, Marinescu D, Shao W, Kahouli I, Prakash S (2014) Enrichment of Bifidobacterium longum subsp. infantis ATCC 15697 within the human gut microbiota using alginate-poly-L-lysine-alginate microencapsulation oral delivery system: an in vitro analysis using a computer-controlled dynamic human gastrointestinal model. J Microencapsul 31:230–238
Darquy S, Reach G (1985) Immunoisolation of pancreatic B cells by microencapsulation. An in vitro study. Diabetologia 28:776–780
Lanza RP, Sullivan SJ, Chick WL (1992) Islet transplantation with immunoisolation. Diabetes 41:1503–1510
Arshady R (1989) Preparation of microspheres and microcapsules by interfacial polycondensation techniques. J Microencapsul 6:13–28
Fritschy WM, Woltens GHJ, Schilfgaarde RV (1991) Effect of alginage-polylysine-alginate microencapsulation on in vitro insulin release from rat pancreatic islets. Diabetes 40:37–43
Strand BL, Gåserød O, Kulseng B, Espevik T, Skjåk-Baek G (2002) Alginate-polylysine-alginate microcapsules: effect of size reduction on capsule properties. J Microencapsul 19:615–630
Huang X, Zhang X, Wang X, Wang C, Tang B (2012) Microenvironment of alginate-based microcapsules for cell culture and tissue engineering. J Biosci Bioeng 114:1–8
Chicheportiche D, Reach G (1988) In vitro kinetics of insulin release by microencapsulated rat islets: effect of the size of the microcapsules. Diabetologia 31:54–57
Robitaille R, Pariseau JF, Leblond F, Lamoureux M, Lepage Y, Halle JP (1999) Studies on small (<350 μm) alginate-poly-l-lysine microcapsules. III. Biocompatibility of smaller versus standard microcapsules. J Biomed Mater Res 44:116–120
Goosen MFA, Al-Ghafri AS, ElMardi O, Al-Belushi MIJ, Al-Hajri HA, Mahmoud ESE, Consolacion EC (1997) Electrostatic droplet generation for encapsulation of somatic tissue: assesment of high-voltage power supply. Biotechnol Prog 13:497–502
Halle JP, Leblond FA, Pariseau JF, Jutras P, Brabant MJ, Lepage Y (1994) Studies on small (<300 μm) microcapsules: II-parameters governing the production of alginate beads by high voltage electrostatic pulses. Cell Transplant 3:365–372
Lacy PE, Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16:35–39
O’Shea GM, Sun AM (1986) Encapsulation of rat islets of Langerhans prolongs xenograft survival in diabetic mice. Diabetes 35:943–946
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Hsu, B.RS., Fu, SH. (2017). Field Effect Microparticle Generation for Cell Microencapsulation. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6364-5_4
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6362-1
Online ISBN: 978-1-4939-6364-5
eBook Packages: Springer Protocols