Abstract
Cell encapsulation is an alternative to avoid rejection of grafted tissue, thus bringing an interesting alternative in cell therapy. It is particularly relevant in ailments where only the implant of small quantities of tissues is warranted. In such circumstances, the use of immunosuppressive therapy in patients implanted with tissues from donors is debatable, yet unavoidable at present in order to prevent rejection and/or sensitization of the host to the tissue, in turn jeopardizing the success of successive implants. Hence, a new line of thought, which aims to provide an immunoprivileged site for the grafted tissue, while at the same time insure its nutrition, as well as its survival and continued function, appears as a most attractive possibility. To achieve these goals, cells or tissues harvested for transplant could be encapsulated in biologically compatible matrices. Among the matrices currently in existence, sodium alginate is the most widely used polymer for tissue encapsulation.
In the present chapter, we present a technique used to encapsulate parathyroid tissue, for use as cell transplant therapy in patients with secondary hypoparathyroidism. With this procedure, implanted tissue survives and remains functional for up to 18 months.
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References
Gac P, Cabané P, Amat J, Huidobro F, Rossi R, Rodríguez F, Ferrada C et al (2007) Incidencia de hipocalcemia pos tiroidectomía total. Rev Med Chil 135(1):26–30
Cusano NE, Rubin MR, McMahon DJ, Zhang C, Ives R, Tulley A, Bilezikian JP (2012) Therapy of hypoparathyroidism with PTH (1–84): a prospective four-year investigation of efficacy and safety. J Clin Endocrinol Metab 98(1):137–144
Hasse C, Klöck G, Schlosser A et al (1997) Parathyroid allo-transplantation without immunosuppression. Lancet 350:1296–1297
Tolloczko T, Wozniewicz B, Sawicki A, Gòrski A (1997) Allotransplantation of cultured human parathyroid cells: present status and perspectives. Transplant Proc 29:998–1000
Nawrot I, Wozniewicz B, Tolloczko T, Sawicki A, Gorski A, Chudzinski W et al (2007) Alotransplantation of cultured parathyroid progenitor cells without immunosuppression: clinical results. Transplantation 83(6):734–740
Bingham EL, Cheng SP, Woods Ignatoski KM, Doherty GM (2009) Differentiation of human embryonic stem cells to a parathyroid-like phenotype. Stem Cells Dev 18(7):1071–1080
Karaöz E, Arıkan S, Aksoy A, Ünal ZS, Adaş M, Adaş G, Duruksu G (2013) Stromal stem cells from parathyroid glands of patients with secondary hyperparathyroidism demonstrate higher telomerase activity and osteogenic differentiation ability than normal bone marrow derived stromal stem cells. Br J Medicine Medical Rese 3(3):654–680
Flechner SM, Berber E, Askar M, Stephany B, Agarwal A, Milas M (2010) Allotransplantation of Cryopreserved Parathyroid Tissue for Severe Hypocalcemia in a Renal Transplant Recipient. Am J Transplant 10:2061–2065
Wilson JL, Najia MA, Saeed R, McDevitt TC (2014) Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates. Biotechnol Bioeng 111(3):618–631
Schneider S, Feilen P, Cramer H, Hillgartner M, Brunnenmeier F, Zimmermann H et al (2003) Beneficial effects of human serum albumin on stability and functionality of alginate microcapsules fabricated in different ways. J Microencapsul 20:627–636
Cabané P, Gac P, Amat J, Pineda P, Rossi R, Caviedes R, Caviedes P (2009) Allotransplant of microencapsulated parathyroid tissue in severe postsurgical hypoparathyroidism: a case report. Transplant Proc 41(9):3879–3883
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Toledo, P.C., Rossi, R.L., Caviedes, P. (2017). Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_27
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DOI: https://doi.org/10.1007/978-1-4939-6364-5_27
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