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Noninvasive Tracking of Alginate-Microencapsulated Cells

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Cell Microencapsulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1479))

Abstract

This chapter presents a description of standardized techniques used routinely in our laboratory to encapsulate different cell types using the alginate-PLL-alginate immunoisolation system. Given the importance of noninvasive tracking of encapsulated cell transplants, we present a detailed guidance to achieve maximum efficiency and functionality of the capsule preparations for optimal tracking posttransplantation. The provided protocols cover tracking of encapsulated cells using magnetic resonance (MR), X-ray, computed tomography (CT), and ultrasound (US) imaging. Practical suggestions to optimize each method with specific references to recommended suppliers are included.

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Author information

Authors and Affiliations

  1. Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Genaro A. Paredes-Juarez & Jeff W. M. Bulte

  2. Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave., Baltimore, MD, 21205, USA

    Genaro A. Paredes-Juarez & Jeff W. M. Bulte

  3. Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Baltimore, MD, 21287-9115, USA

    Brad P. Barnett

  4. Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jeff W. M. Bulte

  5. Department of Chemical & Biomolecular Engineering, The Johns Hopkins Whiting School of Engineering, Baltimore, MD, USA

    Jeff W. M. Bulte

  6. Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jeff W. M. Bulte

Authors
  1. Genaro A. Paredes-Juarez
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  2. Brad P. Barnett
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  3. Jeff W. M. Bulte
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Corresponding author

Correspondence to Jeff W. M. Bulte .

Editor information

Editors and Affiliations

  1. Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine, Winston-Salem, North Carolina, USA

    Emmanuel C. Opara

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Paredes-Juarez, G.A., Barnett, B.P., Bulte, J.W.M. (2017). Noninvasive Tracking of Alginate-Microencapsulated Cells. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6364-5_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6362-1

  • Online ISBN: 978-1-4939-6364-5

  • eBook Packages: Springer Protocols

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