Abstract
A major obstacle to long-term performance of tissue construct implants in regenerative medicine is the inherent hypoxia to which cells in the engineered construct are exposed prior to vascularization of the implant. Various approaches are currently being designed to address this problem. An emerging area of interest on this issue is the use of peroxide-based materials to generate oxygen during the critical period of extended hypoxia that occurs from the time cells are in culture waiting to be used in tissue engineering devices through the immediate post-implant period. In this chapter we provide protocols that we have developed for using these chemical oxygen generators in cell culture and tissue constructs as illustrated by pancreatic islet cell microencapsulation.
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Acknowledgements
The authors would like to acknowledge financial support from the National Institutes of Health (RO1 DK080897) and the Vila Rosenfeld Estate, Greenville NC for the work in Dr. Opara’s laboratory at the Wake Forest Institute for Regenerative Medicine.
Also, research reported in this publication was supported by the National Institute of Biomedical Imaging and Bioengineering (NIBIB) of the National Institutes of Health under award # T32EB014836. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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McQuilling, J.P., Opara, E.C. (2017). Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_10
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DOI: https://doi.org/10.1007/978-1-4939-6364-5_10
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Online ISBN: 978-1-4939-6364-5
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