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Clostridium difficile pp 259–267Cite as

A Practical Method for Preparation of Fecal Microbiota Transplantation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1476))

Abstract

Clostridium difficile is a challenging infection that can be difficult to treat with antibiotic therapy. This chapter outlines the processing material for fecal microbiota transplantation (FMT), also known as stool transplant. Fecal transplantations are effective in treating recurrent C. difficile infection (CDI). FMT uses a stool sample collected from a healthy, screened donor to restore healthy microbiota in the colon of a patient with CDI for symptom resolution. Here, we describe a rapid method for FMT preparation that uses inexpensive and disposable materials.

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Author information

Authors and Affiliations

  1. Department of Medicine, Gastrointestinal Diseases Research Unit, Kingston General Hospital, Queen’s University, Kingston, ON, Canada

    Elizabeth Perez & Elaine O. Petrof

  2. Department of Pathology & Molecular Medicine, St. Joseph’s Healthcare Hamilton, McMaster University, Hamilton, ON, Canada

    Christine H. Lee

Authors
  1. Elizabeth Perez
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  2. Christine H. Lee
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  3. Elaine O. Petrof
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Corresponding author

Correspondence to Elaine O. Petrof .

Editor information

Editors and Affiliations

  1. Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    Adam P. Roberts

  2. Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    Peter Mullany

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© 2016 Springer Science+Business Media New York

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Perez, E., Lee, C.H., Petrof, E.O. (2016). A Practical Method for Preparation of Fecal Microbiota Transplantation. In: Roberts, A., Mullany, P. (eds) Clostridium difficile. Methods in Molecular Biology, vol 1476. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6361-4_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6361-4_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6359-1

  • Online ISBN: 978-1-4939-6361-4

  • eBook Packages: Springer Protocols

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