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Tools to Study SUMO Conjugation in Caenorhabditis elegans

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SUMO

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1475))

Abstract

The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. In recent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in the nematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, the dynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and time within living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can be addressed using recombinant purified components of the C. elegans sumoylation machinery, including E3 ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biological role of SUMO in the context of a multicellular organism.

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Acknowledgments

This work was supported by the Wellcome Trust (grant 098391/Z/12/7), Cancer Research UK (grant C434/A13067), BBSRC (grant BB/J015199/1), and a Marie Curie Fellowship (grant 297881). Some nematode strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). R.T.H. is a Senior Investigator of the Wellcome Trust. Wellcome Trust grant 097045/B/11/Z provided infrastructure support. The author(s) would like to acknowledge networking support by the Proteostasis COST action (BM1307).

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Authors and Affiliations

  1. Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Sir James Black Centre, Dow Street, Dundee, DD1 5EH, UK

    Federico Pelisch & Ronald T. Hay

Authors
  1. Federico Pelisch
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  2. Ronald T. Hay
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Correspondence to Federico Pelisch .

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Editors and Affiliations

  1. INBIOMED, San Sebastian, Spain

    Manuel S. Rodriguez

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© 2016 Springer Science+Business Media New York

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Pelisch, F., Hay, R.T. (2016). Tools to Study SUMO Conjugation in Caenorhabditis elegans . In: Rodriguez, M. (eds) SUMO. Methods in Molecular Biology, vol 1475. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6358-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6358-4_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6356-0

  • Online ISBN: 978-1-4939-6358-4

  • eBook Packages: Springer Protocols

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