Abstract
Polyclonal antibodies consist of a mixture of antibodies produced by multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
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© 2016 Springer Science+Business Media New York
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Nakazawa, M., Mukumoto, M., Miyatake, K. (2016). Production and Purification of Polyclonal Antibodies. In: Schwartzbach, S., Skalli, O., Schikorski, T. (eds) High-Resolution Imaging of Cellular Proteins. Methods in Molecular Biology, vol 1474. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6352-2_3
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DOI: https://doi.org/10.1007/978-1-4939-6352-2_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6350-8
Online ISBN: 978-1-4939-6352-2
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